Uricult Vet for the USA
For vets Aidian offers Uricult Vet for the USA diagnostic test.
Generally
The Uricult Vet dipslide system is based on two culture media for detection of microbes causing urinary tract infections in animals. One side of the plastic slide is covered with green CLED medium and the other side with reddish EMB medium. The CLED medium is intended for determining the total microbial count. The selective EMB medium is intended for detecting gram-negative microbes.
Technical data
| Products available |
|
| Use | For in vitro diagnostic use |
| Method | Culture |
| Sample type | Urine |
| Time to result | 16 - 24 hours |
| Reading of the result | Visual |
| Storage | Room temperature, 45 - 77 °F (7 - 25 °C) |
| Additionally needed | Incubator 94 - 100 °F (36 °C +/- 2 °C); Sterile urine collection cup |
| Country of origin | Finland |
| Registered trademark | Uricult is a registered trademark of Aidian Oy |
About the tests
UTI is frequently found in cats and dogs. Clinical signs are not always present and the symptoms are nonspecific1,2. Symptoms should not be used alone for diagnosis of UTI. When diagnosing UTI in animals, it is recommended to interpret the clinical evaluation, gross and cytological appearance of the urine, and bacterial culture.
The Uricult tests are dipslides for culturing urine samples. The slides are covered on both sides with agar media. The slide can be dipped into urine, or urine can be poured onto the slide. Cystocentesis sample can also be used by placing drops directly from the syringe onto the agar medium. Thereafter, the inoculated slide is inserted back into the tube, making it ready for incubation or for transportation. After incubation, the presence of bacteria is evidenced by colonies growing on the agar surface. The number of colonies indicates the number of colony forming units (CFU/ml) in the urine sample because each colony is the result of multiplication of a single bacterial cell.
The total colony count on Uricult Vet test is determined from the originally green CLED agar by matching the colony density with the model chart. The bacteria may be classified as lactose fermenting or non-lactose fermenting bacteria based on their appearance on CLED agar. The number of gram-negative bacteria can be determined on the Uricult Vet test from the originally reddish, selective EMB agar.
References
- Weese JS, Blondeau JM, Boothe D et al. Antimicrobial use guidelines for treatment of urinary tract disease in dogs and cats: antimicrobial guidelines working group of the international society for companion animal infectious diseases. Veterinary Medicine International, 2011; article ID: 263768. doi:10.4061/2011/263768
- Bartges JW. Diagnosis of urinary tract infections. Vet Clin Small Anim, 2001; 34: 823-933.
Documents and materials
Instructions for use
(For informative use only. Kindly always refer to the latest package insert in the kit.)